5t2h_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE HUMAN TCRA GENE; HARBORS 43 POINT MUTATIONS RELATIVE TO WILD-TYPE I-ONUI (I-ONUI_E-HTCRA)
reference	Stoddard et al. 'Nucleic 45 8621 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.52, R-factor=0.217)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t2h_A interface=23,25,31,33,35,37,39,41,65,69,71,73,75,173,177,179,188,192,196,218,220,222,225,227,229,231,233, ssinpwiltgfadaegsfildiRnRnnesnRyRtSlRfQiTlhnkdksileniqstwkvgkitnSsdrAvMlRvTrfedlkviidhfekyplitqklgdyklfkqafsvmenkehlkengikelvrikakmnwglndelkkafpeniskerplinknipnfkwlagftagegHfgvNlKkvkgtakvYvglRfaiSqhirdknlmnslitylgcgsiReKnKseFrWlEfEvTkfsdindkiipvfqentligvkledfedwckvaklieekkhltesgldeikkiklnmnkgrvf
interface signature	RRRRSRQTSAMRTHNKYRSRKKFWEET

Estimated binding specificities ?

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# IC=22.338 IC/col=1.015 n_of_columns=22

specificity:

A |  80  24  61  96   0   0   0   0   0   0  96  24   0  23  69   7   0  96   0  75   0  96
C |  10  24  11   0   0  96   0   0   0   0   0  24   0   7   0   3  96   0   0   7  96   0
G |   3  24   7   0   0   0  96  96   0  96   0  24   0  53  27  86   0   0  96   7   0   0
T |   3  24  17   0  96   0   0   0  96   0   0  24  96  13   0   0   0   0   0   7   0   0

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Dendrogram of similar interfaces ?

matrix format

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updated Sat Jan 10 08:52:11 2026