5t2n_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE ANOPHELES AGAP007280 GENE; HARBORS 38 POINT MUTATIONS RELATIVE TO WILD-TYPE I- ONUI (I-ONUI_E-AG007820)
reference	Stoddard et al. 'Nucleic 45 8621 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.08, R-factor=0.223)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t2n_A interface=16,18,20,22,27,32,34,38,40,62,64,70,72,74,132,170,174,176,178,187,193,213,215,217,219,222,224,228,230, sinpwiltgfadaegSfIlRiRnnngMrvgyLtElifSiKlhnkdksileniqstwkvgkiTnNgdqavMlRvSrfedlkviidhfekyplitqklgdyklfkqaysvmenkehlkengikelvrikakmnWgltdelkkafpeiierplinknipnlkwlagftsgegHfgvNlWkRkggthvgvQlvfgiSqhirdknlmnslitylgcgYiLeKnRseLsWldfRvTkfsdindkiipvfqentligvkledfedwckvaklieekkhltesgldeikkiklnmnkgr
interface signature	SIRRMLESKTNMRSWHNWRQSYLKRLWRT

Estimated binding specificities ?

readout + contact

# IC=20.505 IC/col=1.025 n_of_columns=20

specificity:

A |   6   0   0   0   1  96   0   6  24   0   0   0   8   0   0  29   0  87   0   4
C |   4  96  96   0  85   0  96  12  24   0  96   0   4  96  96   6  32   2  96  87
G |   8   0   0   0   0   0   0   4  24   0   0   0  12   0   0   4   0   5   0   3
T |  78   0   0  96  10   0   0  74  24  96   0  96  72   0   0  57  64   2   0   2

scan!

Dendrogram of similar interfaces ?

matrix format

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updated Mon Jun 29 21:00:41 2026