5t2n_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE ANOPHELES AGAP007280 GENE; HARBORS 38 POINT MUTATIONS RELATIVE TO WILD-TYPE I- ONUI (I-ONUI_E-AG007820)
reference	Stoddard et al. 'Nucleic 45 8621 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.08, R-factor=0.223)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t2n_A interface=16,18,20,22,27,32,34,38,40,62,64,70,72,74,132,170,174,176,178,187,193,213,215,217,219,222,224,228,230, sinpwiltgfadaegSfIlRiRnnngMrvgyLtElifSiKlhnkdksileniqstwkvgkiTnNgdqavMlRvSrfedlkviidhfekyplitqklgdyklfkqaysvmenkehlkengikelvrikakmnWgltdelkkafpeiierplinknipnlkwlagftsgegHfgvNlWkRkggthvgvQlvfgiSqhirdknlmnslitylgcgYiLeKnRseLsWldfRvTkfsdindkiipvfqentligvkledfedwckvaklieekkhltesgldeikkiklnmnkgr
interface signature	SIRRMLESKTNMRSWHNWRQSYLKRLWRT

Estimated binding specificities ?

readout + contact

# IC=21.037 IC/col=1.052 n_of_columns=20

specificity:

A |   6   0   3  24  85   0   0  72  96   0  96  24  75   0   0  13  96   0   0  85
C |   5   0   3   0   1   0   0   3   0   0   0  24   6   0   0   0   0   0   0   5
G |  82  96   6  72  10  96  96  10   0  96   0  24   6  96   0  82   0  96  96   3
T |   3   0  84   0   0   0   0  11   0   0   0  24   9   0  96   1   0   0   0   3

scan!

Dendrogram of similar interfaces ?

matrix format

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updated Sat Jan 10 08:52:37 2026