5t2n_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE ANOPHELES AGAP007280 GENE; HARBORS 38 POINT MUTATIONS RELATIVE TO WILD-TYPE I- ONUI (I-ONUI_E-AG007820)
reference	Stoddard et al. 'Nucleic 45 8621 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.08, R-factor=0.223)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t2n_A interface=16,18,20,22,27,32,34,38,40,62,64,70,72,74,132,170,174,176,178,187,193,213,215,217,219,222,224,228,230, sinpwiltgfadaegSfIlRiRnnngMrvgyLtElifSiKlhnkdksileniqstwkvgkiTnNgdqavMlRvSrfedlkviidhfekyplitqklgdyklfkqaysvmenkehlkengikelvrikakmnWgltdelkkafpeiierplinknipnlkwlagftsgegHfgvNlWkRkggthvgvQlvfgiSqhirdknlmnslitylgcgYiLeKnRseLsWldfRvTkfsdindkiipvfqentligvkledfedwckvaklieekkhltesgldeikkiklnmnkgr
interface signature	SIRRMLESKTNMRSWHNWRQSYLKRLWRT

Estimated binding specificities ?

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# IC=20.265 IC/col=1.013 n_of_columns=20

specificity:

A |   4   0   0   0   1  96   0   7  24   0   0   0   9   0   0  35   0  87   0   2
C |   5  96  96   0  88   0  96   9  24   0  96   0   7  96  96   5  60   2  96  80
G |   7   0   0   0   0   0   0   7  24   0   0   0   7   0   0   6   1   5   0   7
T |  80   0   0  96   7   0   0  73  24  96   0  96  73   0   0  50  35   2   0   7

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Dendrogram of similar interfaces ?

matrix format

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updated Sun Aug 3 09:59:34 2025