5t2o_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE ANOPHELES AGAP011377 GENE; HARBORS 53 POINT MUTATIONS RELATIVE TO WILD-TYPE I- ONUI (I-ONUI_E-AG011377)
reference	Stoddard et al. 'Nucleic 45 8621 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.80, R-factor=0.230)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t2o_A interface=17,19,21,33,35,37,63,65,69,71,73,133,173,179,185,187,189,191,215,217,219,222,224, esinpwiltgfadaegSfSlYirntndrpsryEtRlTfaiglhykdksileniqstwkvgiiTnAgnnVvQlRvsrfedlkviidhfekyplitqklgdyklfkqafsvmenkehlkengikelvrikakmnWglndelkkafpeniskerplinknipnlkwlagftsgegYfgvilAkaatvQvRlRfEigqhirdknlmnslitylgcghiYeKnKsgRsWlvytvtkfsdisdkiipvfqkntligvkledfedwckvaklieekkhltesgldeikkiklnmnkgrvf
interface signature	SSYERTTAVQRWYAQRREYKKRW

Estimated binding specificities ?

readout + contact

# IC=18.725 IC/col=0.986 n_of_columns=19

specificity:

A |  12  96   0  96   0   0   0  24   0  96  24  24  12   0   0   0   0   0   0
C |  60   0   0   0  96   0   0  24   0   0  24  24  12   0   0   0  96  96   0
G |  12   0  24   0   0  96   0  24  96   0  24  24  12   0   0   0   0   0  96
T |  12   0  72   0   0   0  96  24   0   0  24  24  60  96  96  96   0   0   0

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Dendrogram of similar interfaces ?

matrix format

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updated Sat Jan 10 08:53:03 2026