1lwt_A : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF THE INTEIN HOMING ENDONUCLEASE PI-SCEI BOUND TO ITS SUBSTRATE DNA (CA2+ FREE) (ENDONUCLEASE PI-SCEI)
reference	Quiocho et al.
source	Saccharomyces cerevisiae
experiment	X-ray (resolution=3.20, R-factor=?)
structural superfamily	Hedgehog/intein Hint domain;Homing endonucleases;
sequence family	Hom_endassociated Hint; Homing endonuclease;
redundant complexes	1lws_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 1lwt_A interface=55,88,90,92,94,95,99,101,123,125,127,167,168,171,221,322,334,358,360,363,365,367,369,371,378, cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksQhrahkssrevpellkftcnathelvvrtprsvRrLsRtIKgveYfEvitfemgqkkapdgrivelvkEvSkSypisegperanelvesyrkasnkayfewtieardlsllgSHvrKatyqtyapilyendhffdymqkskfhltiegpkvlayllglwigdglsdRatfsvdsrdtslmervteyaeklnlcaeykdrkaktvnlyskvvrgngirnnlntenplwdaivglgflkdgvknipsflstdnigtretflaglidsdgYvtdehgikatiKtihtsvrdglvslarslglvvsvNaEpaKvDmNgTkHkisyaiYmsggdvllnvlskcagskkfrpapaaafarecrgfyfelqelkeddyygitlsddsdhqfllanqvvvhn
interface signature	QRLRIKYEESSSHKRYKNEKDNTHY

Estimated binding specificities ?

readout + contact

# IC=20.145 IC/col=0.746 n_of_columns=27

specificity:

A |   0   0  84  65   0  24  19  24  24   0   5  24  24   6   1   0   0   0   0  86  24  24   0  24  96   0  92
C |  21  96   3   0   0  24  55  24  24  96   1  24  24  82   1  96  96   1  96   1  24  24   0  24   0  96   2
G |   0   0   8   1   0  24  19  24  24   0  15  24  24   3   1   0   0  65   0   8  24  24   0  24   0   0   1
T |  75   0   1  30  96  24   3  24  24   0  75  24  24   5  93   0   0  30   0   1  24  24  96  24   0   0   1

scan!

Dendrogram of similar interfaces ?

matrix format

  
  QGRALARGITKAYTETECSTSTSTHTKGRG------YA----KG------NTECKCDGNCTTHG--YT   L          +---------------------1lwt_A    
                                                                             +------2 
  ------------------------------QGEC--------QC------------------------   L   !      !       +-------------3c0w_A    
                                                                           --3      +-------1 
  --------------------------------TGQG--RGRGWC--------------------VC--   L   !              +-------------2vs7_A    
                                                                             ! 
  --------------------------------------------KGCGFT------------------   L   +----------------------------2bop_A

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updated Mon Jun 29 14:25:09 2026