1lwt_A : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF THE INTEIN HOMING ENDONUCLEASE PI-SCEI BOUND TO ITS SUBSTRATE DNA (CA2+ FREE) (ENDONUCLEASE PI-SCEI)
reference	Quiocho et al.
source	Saccharomyces cerevisiae
experiment	X-ray (resolution=3.20, R-factor=?)
structural superfamily	Hedgehog/intein Hint domain;Homing endonucleases;
sequence family	Hom_endassociated Hint; Homing endonuclease;
redundant complexes	1lws_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 1lwt_A interface=55,88,90,92,94,95,99,101,123,125,127,167,168,171,221,322,334,358,360,363,365,367,369,371,378, cfakgtnvlmadgsiecienievgnkvmgkdgrpreviklprgretmysvvqksQhrahkssrevpellkftcnathelvvrtprsvRrLsRtIKgveYfEvitfemgqkkapdgrivelvkEvSkSypisegperanelvesyrkasnkayfewtieardlsllgSHvrKatyqtyapilyendhffdymqkskfhltiegpkvlayllglwigdglsdRatfsvdsrdtslmervteyaeklnlcaeykdrkaktvnlyskvvrgngirnnlntenplwdaivglgflkdgvknipsflstdnigtretflaglidsdgYvtdehgikatiKtihtsvrdglvslarslglvvsvNaEpaKvDmNgTkHkisyaiYmsggdvllnvlskcagskkfrpapaaafarecrgfyfelqelkeddyygitlsddsdhqfllanqvvvhn
interface signature	QRLRIKYEESSSHKRYKNEKDNTHY

Estimated binding specificities ?

readout + contact

# IC=20.077 IC/col=0.744 n_of_columns=27

specificity:

A |   0   0  82  46   0  24  24  24  24   0   0  24  24   1   1   0   0   1   0  86  24  24   0  24  96   0  89
C |  23  96   6   1   0  24  59  24  24  96   3  24  24  78   1  96  96   0  96   5  24  24   0  24   0  96   1
G |   1   0   3   0   0  24  13  24  24   0  13  24  24  12   1   0   0  71   0   5  24  24   0  24   0   0   3
T |  72   0   5  49  96  24   0  24  24   0  80  24  24   5  93   0   0  24   0   0  24  24  96  24   0   0   3

scan!

Dendrogram of similar interfaces ?

matrix format

  
  QGRALARGITKAYTETECSTSTSTHTKGRG------YA----KG------NTECKCDGNCTTHG--YT   L          +---------------------1lwt_A    
                                                                             +------2 
  ------------------------------QGEC--------QC------------------------   L   !      !       +-------------3c0w_A    
                                                                           --3      +-------1 
  --------------------------------TGQG--RGRGWC--------------------VC--   L   !              +-------------2vs7_A    
                                                                             ! 
  --------------------------------------------KGCGFT------------------   L   +----------------------------2bop_A

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updated Fri Sep 13 15:48:52 2024