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structure namePURINE REPRESSOR-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
(PROTEIN PURINE NUCLEOTIDE SYNTHESIS REPRESSOR)
referenceSchumacher et al.
sourceEscherichia coli
experimentX-ray (resolution=2.50, R-factor=?)
structural superfamilyPeriplasmic binding protein-like I;lambda repressor-like DNA-binding domains;
sequence familyPeriplasmic binding proteins and sugar b; Bacterial regulatory proteins, lacI fami;
redundant complexes2pua 1bdh 2pub 2puc 2pud 1bdi 2pue 2puf 2pug 1jfs 1jft 1jh9 1qp0 1qp4 1pnr 1vpw 1zay 1qp7 1qqa 1qqb 1wet
reference complex1jft_A
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureSTRLK

Estimated binding specificities ?

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# IC=5.903 IC/col=0.738 n_of_columns=8 specificity:   
A |   0   0  12  38  24  96   0  14
C |  96   0  60  19  24   0  96  14
G |   0  96  12  19  24   0   0  54
T |   0   0  12  20  24   0   0  14
scan!

Related DNA sequences reported in the literature ?


sitesourcematches (E-value)
term: PURINE REPRESSOR
TCGCGAAGAAGAAGAAGAACGCTPubMed1wet_A(2.67e-03), 1jh9_A(2.67e-03), 1pnr_A(2.67e-03), ... (total=4)
CGCACPubMed1qpz_A(1.78e-03), 2pud_A(1.97e-03), 2pue_A(1.77e-03), ... (total=14)
AGCGTTCTTCTTCTTCTTCGCGAPubMed1wet_A(2.67e-03), 1jh9_A(2.67e-03), 1pnr_A(2.67e-03), ... (total=4)
ACGCAAACGTTTGCGTPubMed1qpz_A(2.53e-07), 2pud_A(2.90e-07), 2pue_A(1.63e-04), ... (total=17)
GCGCAAACGTTTTCGTPubMed1qpz_A(2.53e-07), 2pud_A(2.90e-07), 2pue_A(1.63e-04), ... (total=18)
GAAACGPubMed1qpz_A(6.38e-03), 2pud_A(6.95e-03), 1jfs_A(7.13e-03), ... (total=12)

Dendrogram of similar interfaces ?

matrix format
  
  ----SG--TC------RG------LCKA   L             +1qpz_A    
                                     +---------2 
  ITYTSGMTAA--STRG--NT--AGLCAT   L   !         ! +3oqm_C    
                                   --3         +-1 
  IT--ST--ATTTSTRG----VTAGLCAC   L   !           +1rzr_C    
                                     ! 
  LTYCSGYGQC--STRG--HT--AGLGAC   L   +----------1efa_B    
                                   

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updated Thu Sep 12 15:22:54 2024