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structure namePURINE REPRESSOR-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
(PROTEIN PURINE NUCLEOTIDE SYNTHESIS REPRESSOR)
referenceSchumacher et al.
sourceEscherichia coli
experimentX-ray (resolution=2.50, R-factor=?)
structural superfamilyPeriplasmic binding protein-like I;lambda repressor-like DNA-binding domains;
sequence familyPeriplasmic binding proteins and sugar b; Bacterial regulatory proteins, lacI fami;
redundant complexes2pua 1bdh 2pub 2puc 2pud 1bdi 2pue 2puf 2pug 1jfs 1jft 1jh9 1qp0 1qp4 1pnr 1vpw 1zay 1qp7 1qqa 1qqb 1wet
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureSTRLK

Estimated binding specificities ?

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# IC=5.903 IC/col=0.738 n_of_columns=8 specificity:   
A |   0   0  12  38  24  96   0  14
C |  96   0  60  19  24   0  96  14
G |   0  96  12  19  24   0   0  54
T |   0   0  12  20  24   0   0  14
scan!

Related DNA sequences reported in the literature ?


sitesourcematches (E-value)
term: PURINE REPRESSOR
TCGCGAAGAAGAAGAAGAACGCTPubMed1wet_A(2.67e-03), 1jh9_A(2.67e-03), 1pnr_A(2.67e-03), ... (total=4)
CGCACPubMed1qpz_A(1.78e-03), 2pud_A(1.97e-03), 2pue_A(1.77e-03), ... (total=14)
AGCGTTCTTCTTCTTCTTCGCGAPubMed1wet_A(2.67e-03), 1jh9_A(2.67e-03), 1pnr_A(2.67e-03), ... (total=4)
ACGCAAACGTTTGCGTPubMed1qpz_A(2.53e-07), 2pud_A(2.90e-07), 2pue_A(1.63e-04), ... (total=17)
GCGCAAACGTTTTCGTPubMed1qpz_A(2.53e-07), 2pud_A(2.90e-07), 2pue_A(1.63e-04), ... (total=18)
GAAACGPubMed1qpz_A(6.38e-03), 2pud_A(6.95e-03), 1jfs_A(7.13e-03), ... (total=12)

Dendrogram of similar interfaces ?

matrix format
  
  ----SG--TC------RG------LCKA   L             +1qpz_A    
                                     +---------2 
  ITYTSGMTAA--STRG--NT--AGLCAT   L   !         ! +3oqm_C    
                                   --3         +-1 
  IT--ST--ATTTSTRG----VTAGLCAC   L   !           +1rzr_C    
                                     ! 
  LTYCSGYGQC--STRG--HT--AGLGAC   L   +----------1efa_B    
                                   

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updated Mon Dec 16 18:41:23 2024