2h27_D : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF ESCHERICHIA COLI SIGMAE REGION 4 BOUND TO ITS-35 ELEMENT DNA (RNA polymerase Sigma E factor)
reference	Darst et al. 'Plos 4 e269 2006
source	Escherichia coli K12
experiment	X-ray (resolution=2.30, R-factor=0.241)
structural superfamily	Sigma3 and sigma4 domains of RNA polymerase sigma factors;
sequence family	Sigma70, region 4;
reference complex	4lup_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 2h27_D interface=35,48,50,51,54,55, mlseelrqivfrtieslpedlrmaitlreldglsYeeiaaimdcpvgTvRSriFRareaidnkvqplir
interface signature	YTRSFR

Estimated binding specificities ?

readout + contact

# IC=6.610 IC/col=0.944 n_of_columns=7

specificity:

A |   8   0   8   0   0   0   8
C |   8   0   8   0  88  96   8
G |  69  96   8   0   0   0  72
T |  11   0  72  96   8   0   8

scan!

Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: SIGMAE
GGAACTT	PubMed	2h27_D(2.47e-08)

Dendrogram of similar interfaces ?

matrix format

  
  --YG------TGRGSGFTRG------   L                       +----2h27_D    
                                                 +-----2 
  ----TTRG--ATSTQT--RG------   L                 !     +----2w7n_A    
                                   +-------------3 
  ----TTRGECRTRCQT----------   L   !             !     +----1rio_H    
                                 --4             +-----1 
  SCRC----RCTT--VTRGRG------   L   !                   +----1gxp_B    
                                   ! 
  ------------RTRA--TAEGWANG   L   +------------------------2p6r_A

home
updated Thu Jan 8 20:05:13 2026