interface graph thumbnail
legend  pdf

structure nameCRYSTAL STRUCTURE OF THE COMPLEX FORMED BY REGION OF E. COLI SIGMAE BOUND TO ITS -10 ELEMENT NON TEMPLATE STRAND
(RNA polymerase sigma factor)
referenceVorholt et al. Nat.Struct.Mol.Biol. 21 269 2014
sourceEscherichia coli
experimentX-ray (resolution=1.20, R-factor=0.147)
structural superfamilySigma2 domain of RNA polymerase sigma factors;
sequence familySigma70 region 2;
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureKAFRDSYTWYRINN

Estimated binding specificities ?

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# IC=5.853 IC/col=0.836 n_of_columns=7 specificity:   
A |  14   0   0   0  24  60  96
C |  14   0   0  96  24  12   0
G |  14  96   0   0  24  12   0
T |  54   0  96   0  24  12   0
scan!

Related DNA sequences reported in the literature ?


sitesourcematches (E-value)
term: SIGMAE
GTCTAAPubMed4lup_A(6.24e-06)
TCTAAPubMed4lup_A(1.00e-03)

Dendrogram of similar interfaces ?

matrix format
  
  --------SC------PTSC--KTATSCFCRC--DC------TAWTYARTIT--NT--KCNC--VTRA--   L       +-----------------------------6jbq_F    
                                                                                 +-1 
  IA--LAKARAFT--YTTT--WT------FA--KAYA----HA--YATAQAAT--NT--VA--IT----VT   L   +-2 +-----------------------------7d7c_F    
                                                                               ! ! 
  --DGQG----LGMART--PG--KT----FTRT----TTNTKGAGWTYGRTITTGNTYAICNCYA--RG--     --3 +-----------------------------5zx2_F    
                                                                               ! 
  --DG------LG------PG--KT----FTRT----TTNT--AGWTYGRTITTGNT--------------       +-----------------------------6jcy_F    
                                                                             

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updated Fri Sep 13 18:29:31 2024