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structure nameSTRUCTURE OF RESTRICTION ENDONUCLEASE BSTYI BOUND TO NON-COGNATE DNA
(BSTYI)
referenceSamuelson et al. Structure 15 449 2007
sourceGEOBACILLUS STEAROTHERMOPHILUS
experimentX-ray (resolution=2.10, R-factor=?)
structural superfamilyRestriction endonuclease-like;
sequence familyRestriction endonuclease BglII;
multimeric complexes1vrr_AB 2p0j_AB
redundant complexes1vrr_B
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureEKYS

Estimated binding specificities ?

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# IC=3.101 IC/col=0.775 n_of_columns=4 specificity:   
A |  16   0   0  54
C |  13  96  96  13
G |  13   0   0  13
T |  54   0   0  16
scan!

Related DNA sequences reported in the literature ?


sitesourcematches (E-value)
term: BSTYI
AGATCTPubMed1vrr_AB(2.56e-08), 1vrr_A(7.01e-03)
GGATCTPubMed1vrr_AB(6.82e-05), 1vrr_A(7.01e-03)
AGATCCPubMed1vrr_AB(6.82e-05), 1vrr_A(7.01e-03)

Dendrogram of similar interfaces ?

matrix format
  
  EC--KGYT------------SC----   L   +----------2p0j_A    
                                 --3 
  QTSANTYAFG--------STNTSAST   L   ! +--------1dfm_A    
                                   +-2 
  ----NG----RGKT--YTTTDCRGVT   L     !   +----3bam_A    
                                     +---1 
  ----NG----RGRTYT--TTDCRG--   L         +----3odh_F    
                                 

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updated Fri Sep 13 16:34:38 2024