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structure nameI-SCEI MUTANT (K86R/G100T)COMPLEXED WITH C/G+4 DNA SUBSTRATE
(Intron encoded endonuclease I-SceI)
referenceGolden et al. J.Mol.Biol. 405 185 2011
sourceSaccharomyces cerevisiae
experimentX-ray (resolution=2.50, R-factor=0.238)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG DNA endonuclease family;
reference complex1r7m_B
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signaturePNYRRQERRNHVQWDYNNQNK

Estimated binding specificities ?

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# IC=18.219 IC/col=0.959 n_of_columns=19 specificity:   
A |   2   0  93  83   0   0  72  24  24  80   0   0   0   0   0   6  90  85   1
C |  75   0   2   0   0   0   2  24  24   2  96  96   0   0   0   2   2   2  10
G |  12   0   1  10  80  96  15  24  24   2   0   0  96  96  96   0   4   2   2
T |   7  96   0   3  16   0   7  24  24  12   0   0   0   0   0  88   0   7  83
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updated Thu Jan 8 22:29:04 2026