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structure nameI-SCEI MUTANT (K86R/G100T)COMPLEXED WITH C/G+4 DNA SUBSTRATE
(Intron encoded endonuclease I-SceI)
referenceGolden et al. J.Mol.Biol. 405 185 2011
sourceSaccharomyces cerevisiae
experimentX-ray (resolution=2.50, R-factor=0.238)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG DNA endonuclease family;
reference complex1r7m_B
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signaturePNYRRQERRNHVQWDYNNQNK

Estimated binding specificities ?

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# IC=18.278 IC/col=0.962 n_of_columns=19 specificity:   
A |  86   6   3  86   0   0   0   0   0  30  24  24   3   0   9   0   0  96   6
C |   0   6   3   6  96  96  96   0   0   5  24  24   7  96  86  10   0   0   6
G |   7   4   3   0   0   0   0  96  96   3  24  24   3   0   1   0   0   0  80
T |   3  80  87   4   0   0   0   0   0  58  24  24  83   0   0  86  96   0   4
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updated Sun Jun 28 08:04:05 2026