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structure nameTHE HOMING ENDONUCLEASE I-SCEI BOUND TO ITS DNA RECOGNITION REGION
(Intron-encoded endonuclease I-SceI)
referenceQuiocho et al. J.Mol.Biol. 334 685 2003
sourceSaccharomyces cerevisiae
experimentX-ray (resolution=2.25, R-factor=0.235)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG DNA endonuclease family;
redundant complexes3ool_A 3oor_A
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signaturePNYRRQEKRVQDYNNQNK

Estimated binding specificities ?

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# IC=18.000 IC/col=0.947 n_of_columns=19 specificity:   
A |  94   5   2  76   0   0   0   0   0  16  24  24   2   0   0   8   0  96   8
C |   0  10   8   7  96  96  96   0   0   4  24  24   2  96  96  74   0   0   9
G |   2   5   5   8   0   0   0   0  96   5  24  24   5   0   0   9   0   0  74
T |   0  76  81   5   0   0   0  96   0  71  24  24  87   0   0   5  96   0   5
scan!

Dendrogram of similar interfaces ?

matrix format
  
  PTNTYGRGRGQG--EC----KT--RG--VAQC------DTYCNT--------NCQC----------NGKG------------   L                      +-------------1r7m_B    
                                                                                                     +--------1 
  ----YTST----------EC--RG--RG----CC--ST--YG------------------YA--------CCKT--KG--TT   L         +---2        +-------------2qoj_Z    
                                                                                                 !   ! 
  ----ST------EC--AT----YG--NA----CT----------------------RA--ET--RG----ATDT----TA--   L       +-3   +----------------------4lq0_A    
                                                                                               ! ! 
  --------------------------------YC----------QARTRGEC------YA--KG--KG--RTWC--------   L     +-4 +--------------------------5t2o_A    
                                                                                             ! ! 
  --------------------------------CC------------------------RT------KGRTVAEC--QT----   L   +-5 +---------------------------4yit_A    
                                                                                           ! ! 
  --------------------------------CTVA------------------------ST--DA--RGTC--KT--TG--   L --6 +----------------------------5esp_A    
                                                                                           ! 
  --------------------------------HT------------------------YT--QA--YT--RTWCST--ST--   L   +----------------------------5t8d_A    
                                                                                         

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updated Fri Sep 13 15:57:59 2024