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structure nameTHE HOMING ENDONUCLEASE I-SCEI BOUND TO ITS DNA RECOGNITION REGION
(Intron-encoded endonuclease I-SceI)
referenceQuiocho et al. J.Mol.Biol. 334 685 2003
sourceSaccharomyces cerevisiae
experimentX-ray (resolution=2.25, R-factor=0.235)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG DNA endonuclease family;
redundant complexes3ool_A 3oor_A
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signaturePNYRRQEKRVQDYNNQNK

Estimated binding specificities ?

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# IC=17.853 IC/col=0.940 n_of_columns=19 specificity:   
A |  94   2   5  79   0   0   0   0   0  22  24  24   5   0   0   5   2  96  10
C |   0  11   7   4  96  96  96   0   0   5  24  24   2  96  96  81   5   0   5
G |   2   4   5   8   0   0   0   0  96   2  24  24   2   0   0   5   5   0  76
T |   0  79  79   5   0   0   0  96   0  67  24  24  87   0   0   5  84   0   5
scan!

Dendrogram of similar interfaces ?

matrix format
  
  PTNTYGRGRGQG--EC----KT--RG--VAQC------DTYCNT--------NCQC----------NGKG------------   L                    +-------------1r7m_B    
                                                                                                 +----------2 
  ----YTST----------EC--RG--RG----CC--ST--YG------------------YA--------CCKT--KG--TT   L         !          +-------------2qoj_Z    
                                                                                             +---4 
  ----ST------EC--AT----YG--NA----CT----------------------RA--ET--RG----ATDT----TA--   L     !   !                        +4lq0_A    
                                                                                             !   !         +--------------1 
  --------------------------------CC------------------------RT------KGRTVAEC--QT----   L   +-5   +---------3              +4yit_A    
                                                                                           ! !             ! 
  --------------------------------YC----------QARTRGEC------YA--KG--KG--RTWC--------   L   ! !             +--------------5t2o_A    
                                                                                         --6 ! 
  --------------------------------CTVA------------------------ST--DA--RGTC--KT--TG--   L   ! +----------------------------5esp_A    
                                                                                           ! 
  --------------------------------HT------------------------YT--QA--YT--RTWCST--ST--   L   +----------------------------5t8d_A    
                                                                                         

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updated Sat Jan 10 04:54:56 2026