5czz_A : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS CAS9 IN COMPLEX WITH SGRNA AND TARGET DNA (TTGAAT PAM) (CRISPR-associated endonuclease Cas9)
reference	Nishimasu et al. Cell 162 1113 2015
source	Staphylococcus aureus subsp. aureus
experiment	X-ray (resolution=2.60, R-factor=0.213)
structural superfamily	Ribonuclease H-like;
multimeric complexes	8jg9_ACF
redundant complexes	5axw
reference complex	7vw3_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5czz_A interface=876,976,977,980,982,993,1004,1006, rnyilgldigitsvgygiidyetrdvidagvrlfkeanvennegrrskrgarrlkrrrrhriqrvkkllfdynlltdhselsginpyearvkglsqklseeefsaallhlakrrgvhnvneveedtgnelstkeqisrnskaleekyvaelqlerlkkdgevrgsinrfktsdyvkeakqllkvqkayhqldqsfidtyidlletrrtyyegpgegspfgwkdikewyemlmghctyfpeelrsvkyaynadlynalndlnnlvitrdenekleyyekfqiienvfkqkkkptlkqiakeilvneedikgyrvtstgkpeftnlkvyhdikditarkeiienaelldqiakiltiyqssediqeeltnlnseltqeeieqisnlkgytgthnlslkainlildelwhtndnqiaifnrlklvpkkvdlsqqkeipttlvddfilspvvkrsfiqsikvinaiikkyglpndiiielareknskdaqkminemqkrnrqtnerieeiirttgkenakyliekiklhdmqegkclysleaipledllnnpfnyevdhiiprsvsfdnsfnnkvlvkqeeaskkgnrtpfqylsssdskisyetfkkhilnlakgkgrisktkkeylleerdinrfsvqkdfinrnlvdtryatrglmnllrsyfrvnnldvkvksinggftsflrrkwkfkkernkgykhhaedaliianadfifkewkkldkakkvmenqmfmpeieteqeykeifitphqikhikdfkdykyshrvdkkpnrelindtlystrkddkgntlivnnlnglydkdndklkklinkspekllmyhhdpqtyqklklimeqygdeknplykyyeetgnyltkyskkdngpvikkikyygNklnahlditddypnsrnkvvklslkpyrfdvyldngvykfvtvknldvikkenyyevnskayeeakklkkisnqaefiasfynndlikingelyrvigvNNdlLnRievnmidityReylenmndkrPpRiiktiasktqsikkystdilgnlyevkskkhpqiikk
interface signature	NNNLRRPR

Estimated binding specificities ?

readout + contact

# IC=4.475 IC/col=0.895 n_of_columns=5

specificity:

A |  96  13   0   0  57
C |   0  16   0  96  13
G |   0  13   0   0  13
T |   0  54  96   0  13

scan!

Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: CAS9
TTGAAT	PubMed	5czz_A(1.30e-07)
TTGGGT	PubMed	5axw_A(1.30e-07)

Dendrogram of similar interfaces ?

matrix format

  
  ----YTFT--------RG--------------------   L      +------------6rja_C    
                                               +--2 
  LAYA----MTTG--NG--KGDGDCNTRGYG--------   L   !  ! +----------6je3_A    
                                             --3  +-1 
  --YC----LG--NT--------TATTST------KTSG   L   !    +----------5x2h_A    
                                               ! 
  --------RC--------------------RGPTLT--   L   +---------------6joo_A

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updated Fri Jan 9 02:59:35 2026