5t8d_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE HIV INTEGRASE GENE; HARBORS 47 POINT MUTATIONS RELATIVE TO WILD-TYPE I-ONUI (I-ONUI_E-VHIVINT_V2)
reference	Stoddard et al. 'Protein 30 503 2017
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.15, R-factor=0.185)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	5v0q_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5t8d_A interface=18,22,33,35,37,39,59,61,63,67,71,131,171,175,177,181,186,188,190,192,194,216,218,220,223,225,227,229,231,290, assinpwiltgfadaegSfglYiinrniryttRlKfTiTlhnkdksileniqstwkvgIiAnNgdnAvslIvtrfedlkviidhfekyplitqklgdyklfkqafsvmenkehlkengikelvrikakmnWgltdelkkafpgnisrerplinknipnfkwlagftsgegHfgvElIknsRnsrvHvRlRfEiAqhvrdknlmnslitylgcgyiYeQnYseRsWlRfSvSkfsdindkiipvfqentligvkledfedwckvaklieekkhltesgldeikkiklnmnKgr
interface signature	SYRKTTIANAIWHEIRHRREAYQYRWRSSK

Estimated binding specificities ?

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# IC=21.060 IC/col=1.003 n_of_columns=21

specificity:

A |   0   0   5   0  96   0   7  96  24  11   0  96   0   0   0  96   0  96  57  24   7
C |   0   0   3  88   0   0   0   0  24  11  96   0   0  96  96   0  96   0  15  24  10
G |   0  96  19   1   0  96   9   0  24   7   0   0   0   0   0   0   0   0  13  24  76
T |  96   0  69   7   0   0  80   0  24  67   0   0  96   0   0   0   0   0  11  24   3

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Dendrogram of similar interfaces ?

matrix format

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updated Sun Aug 3 10:01:59 2025