6uvw_D : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY (I-ONUI-E-THERM)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.55, R-factor=0.225)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uvw_D interface=18,22,24,26,28,34,36,40,42,66,70,74,173,175,177,179,188,190,196,216,218,220,222,225,227,233, resinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlhnkdksileniqstwgvgvianSgdnAvslKvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnwgltdelkkafpeiskerslinknipnfkwlagftsgegCfFvNlIksksklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWldfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
interface signature	SRRNKSEQTSAKCFNIQQTYKKKFWT

Estimated binding specificities ?

readout + contact

# IC=20.679 IC/col=0.985 n_of_columns=21

specificity:

A |  93  96  96  96  21   8   7  19   0  96  24   0  24  76   0   0   0   0  76  87  82
C |   0   0   0   0   1   1   2   5   0   0  24   0  24   7   0   0   0   0   5   2   7
G |   0   0   0   0  74  87   8   2  96   0  24   0  24   5  96   0  96  96   5   2   2
T |   3   0   0   0   0   0  79  70   0   0  24  96  24   8   0  96   0   0  10   5   5

scan!

home
updated Tue Dec 17 07:27:35 2024