6uvw_D : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY (I-ONUI-E-THERM)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.55, R-factor=0.225)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uvw_D interface=18,22,24,26,28,34,36,40,42,66,70,74,173,175,177,179,188,190,196,216,218,220,222,225,227,233, resinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlhnkdksileniqstwgvgvianSgdnAvslKvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnwgltdelkkafpeiskerslinknipnfkwlagftsgegCfFvNlIksksklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWldfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
interface signature	SRRNKSEQTSAKCFNIQQTYKKKFWT

Estimated binding specificities ?

readout + contact

# IC=22.041 IC/col=1.050 n_of_columns=21

specificity:

A |   8   2   8   0   0  96   0  14  10  96  24   0   0  79  84   0   0   0   0   0   0
C |   2   0   2  96  96   0  96   8   8   0  24   0  96   3   2  88  70   0   0   0   0
G |   2   1   2   0   0   0   0   2  14   0  24   0   0   0   5   0   1   0   0   0   0
T |  84  93  84   0   0   0   0  72  64   0  24  96   0  14   5   8  25  96  96  96  96

scan!

home
updated Fri Jan 9 10:34:04 2026