6uvw_D : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY (I-ONUI-E-THERM)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.55, R-factor=0.225)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uvw_D interface=18,22,24,26,28,34,36,40,42,66,70,74,173,175,177,179,188,190,196,216,218,220,222,225,227,233, resinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlhnkdksileniqstwgvgvianSgdnAvslKvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnwgltdelkkafpeiskerslinknipnfkwlagftsgegCfFvNlIksksklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWldfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
interface signature	SRRNKSEQTSAKCFNIQQTYKKKFWT

Estimated binding specificities ?

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# IC=22.309 IC/col=1.062 n_of_columns=21

specificity:

A |   2   2   5   0   0  96   0   8   7  96  24   0   0  74  82   0   0   0   0   0   0
C |   2   0   7  96  96   0  96   5   8   0  24   0  96   0   7  85  77   0   0   0   0
G |   2   2   2   0   0   0   0   2  16   0  24   0   0   0   2   1   0   0   0   0   0
T |  90  92  82   0   0   0   0  81  65   0  24  96   0  22   5  10  19  96  96  96  96

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updated Fri Sep 13 02:28:47 2024