6uvw_D : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY (I-ONUI-E-THERM)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.55, R-factor=0.225)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uvw_D interface=18,22,24,26,28,34,36,40,42,66,70,74,173,175,177,179,188,190,196,216,218,220,222,225,227,233, resinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlhnkdksileniqstwgvgvianSgdnAvslKvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnwgltdelkkafpeiskerslinknipnfkwlagftsgegCfFvNlIksksklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWldfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
interface signature	SRRNKSEQTSAKCFNIQQTYKKKFWT

Estimated binding specificities ?

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# IC=20.500 IC/col=0.976 n_of_columns=21

specificity:

A |  90  96  96  96  21   5   2  16   0  96  24   0  24  79   0   0   0   0  76  82  84
C |   2   0   0   0   0   1   7   2   0   0  24   0  24   2   3   0   0   0   7   4   5
G |   2   0   0   0  75  90   8   7  96   0  24   0  24   7  93   0  96  96   5   5   5
T |   2   0   0   0   0   0  79  71   0   0  24  96  24   8   0  96   0   0   8   5   2

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updated Sat Aug 2 14:35:05 2025