6uwj_A : 3D-footprint complex

structure name	INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY (I-ONUI-E-THERM-HCHR11V2)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=1.85, R-factor=0.194)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	6uvw_D 6uw0_B 6uwg_A 6uwh_A 6uwk_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwj_A interface=18,20,22,24,26,28,34,36,40,42,44,46,66,72,74,76,134,174,176,178,180,182,184,189,191,197,217,219,221,223,226,228,230,234,293, assinpwiltgfadaegSfLlRiRkNnKssvgyStElgfQiTlHnKdksileniqstwgvgvianSgdnavSlKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlSkKkTklgvQvKlvfsiSqhirdknlmnslitylgcgYiKkKnKseFsWlEfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnK
interface signature	SLRRNKSEQTHKSSKTWCFNSKTQKSYKKKFWETK

Estimated binding specificities ?

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# IC=21.756 IC/col=1.145 n_of_columns=19

specificity:

A |   0  96   0  96   0   0   0   0   0  96  96  24  54  54   0   0   0   0  96
C |   0   0   0   0   0   0   0  96   0   0   0  24  16  13   0   0   0   0   0
G |  96   0  96   0  96  96   0   0  96   0   0  24  13  16  96   0  96  96   0
T |   0   0   0   0   0   0  96   0   0   0   0  24  13  13   0  96   0   0   0

scan!

Dendrogram of similar interfaces ?

matrix format

  
  SGSTLGQG--KTYARGKGVT------HCHG----QTRTDC--SANA--KGSC--ST--TASARGQGRG------YC------RTEAVG   L                                 +4yis_A    
                                                                                                               +---------------1 
  ST--------------RC--ATVC----DC--YARTRGTAWGSA--------FC--RG----RGQG--EASCYT----RTWCQGRTEC   L   +-------------2               +7rcc_D    
                                                                                                 !             ! 
  SGIGQAKGNCSC--RGQG--DGTAYCAT--SCVCKGRG--------------------------------------------------   L --3             +---------------6fb0_A    
                                                                                                 ! 
  ------------------------------------EC--------DT----------------------------NG----------   L   +-----------------------------1r7m_B

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updated Tue Jun 30 01:35:05 2026