6uwj_A : 3D-footprint complex

structure name	INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY (I-ONUI-E-THERM-HCHR11V2)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=1.85, R-factor=0.194)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	6uvw_D 6uwg_A 6uwh_A 6uwk_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwj_A interface=18,20,22,24,26,28,34,36,40,42,44,46,66,72,74,76,134,174,176,178,180,182,184,189,191,197,217,219,221,223,226,228,230,234,293, assinpwiltgfadaegSfLlRiRkNnKssvgyStElgfQiTlHnKdksileniqstwgvgvianSgdnavSlKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlSkKkTklgvQvKlvfsiSqhirdknlmnslitylgcgYiKkKnKseFsWlEfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnK
interface signature	SLRRNKSEQTHKSSKTWCFNSKTQKSYKKKFWETK

Estimated binding specificities ?

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# IC=20.905 IC/col=1.100 n_of_columns=19

specificity:

A |   7   0   0  96   0   9   7  24   0   9   0   0  96   0   0   0   0   0   0
C |   7  96  96   0  96   7   7  24   0   7  96   0   0  96  96   0  96   0  96
G |   9   0   0   0   0   7   9  24   0   7   0  96   0   0   0   0   0   0   0
T |  73   0   0   0   0  73  73  24  96  73   0   0   0   0   0  96   0  96   0

scan!

Dendrogram of similar interfaces ?

matrix format

  
  SA--RARGRGKT----EC--ECKG--STATRGRG--WGCAFT--NT--LA------QACT----SA--YTLAKG--FTWCEA--TT   L                              +6uw0_B    
                                                                                                 +------------------------1 
  SGSTLGQG--KTYARGKGVT----HCHG--QTRTDC--SANA--KGSC--STTASARGQGRG----------YC------RTEAVG   L   +-2                        +4yis_A    
                                                                                               ! ! 
  --------------------------------EC--------DT------------------------------NG----------   L --3 +-------------------------1r7m_B    
                                                                                               ! 
  --------------------------------------------------------------FC--AG--RC--------FA----   L   +---------------------------6dg0_A

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updated Sun Aug 3 13:34:07 2025