6uwj_A : 3D-footprint complex

structure name	INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY (I-ONUI-E-THERM-HCHR11V2)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=1.85, R-factor=0.194)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	6uvw_D 6uwg_A 6uwh_A 6uwk_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwj_A interface=18,20,22,24,26,28,34,36,40,42,44,46,66,72,74,76,134,174,176,178,180,182,184,189,191,197,217,219,221,223,226,228,230,234,293, assinpwiltgfadaegSfLlRiRkNnKssvgyStElgfQiTlHnKdksileniqstwgvgvianSgdnavSlKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlSkKkTklgvQvKlvfsiSqhirdknlmnslitylgcgYiKkKnKseFsWlEfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnK
interface signature	SLRRNKSEQTHKSSKTWCFNSKTQKSYKKKFWETK

Estimated binding specificities ?

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# IC=21.797 IC/col=1.147 n_of_columns=19

specificity:

A |   0  96   0  96   0   0   0   0   0  96  96  24  54  57   0   0   0   0  96
C |   0   0   0   0   0   0   0  96   0   0   0  24  16  13   0   0   0   0   0
G |  96   0  96   0  96  96   0   0  96   0   0  24  13  13  96   0  96  96   0
T |   0   0   0   0   0   0  96   0   0   0   0  24  13  13   0  96   0   0   0

scan!

Dendrogram of similar interfaces ?

matrix format

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updated Fri Sep 13 23:53:40 2024