6uwg_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND E178D MUTATION AT CATALYTIC SITE (I-ONUI-E-THERM-E178D)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.22, R-factor=0.197)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwg_A interface=19,23,25,27,29,35,37,41,43,45,47,67,73,75,77,135,175,177,179,181,183,185,190,192,198,218,220,222,224,227,229,231,235,294, rresinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlHnKdksileniqstwgvgvianSgdnavSlKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlIkSkSklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWlDfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnKgrvf
interface signature	SRRNKSEQTHKSSKTWCFNISSQQTYKKKFWDTK

Estimated binding specificities ?

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# IC=22.427 IC/col=1.180 n_of_columns=19

specificity:

A |   0   0   0  96   0   1   8  69   0   0   0  96  69   0   0   0   0   0   0
C |   0  96  96   0  96   0  11   8   0   0  96   0   8  96  96   0   0   0   0
G |   0   0   0   0   0   8   8   8   0   0   0   0   8   0   0   0   0   0   0
T |  96   0   0   0   0  87  69  11  96  96   0   0  11   0   0  96  96  96  96

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updated Tue Dec 17 07:27:50 2024