6uwg_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND E178D MUTATION AT CATALYTIC SITE (I-ONUI-E-THERM-E178D)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.22, R-factor=0.197)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwg_A interface=19,23,25,27,29,35,37,41,43,45,47,67,73,75,77,135,175,177,179,181,183,185,190,192,198,218,220,222,224,227,229,231,235,294, rresinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlHnKdksileniqstwgvgvianSgdnavSlKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlIkSkSklgvQvQlvfsiTqhirdknlmnslitylgcgYiKkKnKseFsWlDfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnKgrvf
interface signature	SRRNKSEQTHKSSKTWCFNISSQQTYKKKFWDTK

Estimated binding specificities ?

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# IC=21.329 IC/col=1.123 n_of_columns=19

specificity:

A |  96  96  96  96   0   0  11   0   0  96  96  24  69  67   0   0   0   0  96
C |   0   0   0   0   0   0   9   0   0   0   0  24   9   9   0   0   0   0   0
G |   0   0   0   0  96  96   9   0  96   0   0  24   9   9  96   0  96  96   0
T |   0   0   0   0   0   0  67  96   0   0   0  24   9  11   0  96   0   0   0

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updated Sun Jun 28 20:01:39 2026