6uwh_A : 3D-footprint complex

structure name	INTERMEDIATE ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND PARTIALLY ALTERED SPECIFICITY (I-ONUI-E-THERM-HCHR11V1)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.30, R-factor=0.208)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwh_A interface=17,21,23,25,27,33,35,39,41,43,65,71,73,133,173,175,177,179,188,190,196,216,218,220,225,227,229,233,292, ssinpwiltgfadaegSfllRiRkNnKssvgyStElgfQiTlHnkdksileniqstwgvgvianSgdnavSlKvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlIksksklgvQvQlvfsiSqhirdknlmnslitylgcgYiKkKnkseFsWlEfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnKgr
interface signature	SRRNKSEQTHSSKWCFNIQQSYKKFWETK

Estimated binding specificities ?

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# IC=20.737 IC/col=1.091 n_of_columns=19

specificity:

A |   0   0   0  96   0   6   6  24   0   6   0   6  78   0   0   0   0   0   0
C |   0  96  96   0  96   6   6  24   0   6  96   6   6  96  96   0   0   0   0
G |   0   0   0   0   0   6   6  24   0   6   0  78   6   0   0   0   0   0   0
T |  96   0   0   0   0  78  78  24  96  78   0   6   6   0   0  96  96  96  96

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updated Fri Jan 9 10:35:02 2026