6uwk_A : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED STABILITY AND FULLY ALTERED SPECIFICITY TARGETING HUMAN CHROMOSOME 11 TRANS INTEGRATION SITE (I-ONUI-E-THERM-HCHR11V3)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.53, R-factor=0.239)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uwk_A interface=18,22,24,26,28,29,34,36,40,66,74,76,134,174,176,178,180,189,191,197,217,219,221,223,226,228,230,234, assinpwiltgfadaegSfllRiRkYsQTrvgyLtElgfQitlhnkdksileniqstwgvgvianSgdnavslKvTrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgdgCfFvNlSkkktklgvQvKlvfsiSqhirdknlmnslitylgcgYiKkKnKseFsWlEfvvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkg
interface signature	SRRYQTLEQSKTWCFNSQKSYKKKFWET

Estimated binding specificities ?

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# IC=21.104 IC/col=1.055 n_of_columns=20

specificity:

A |   9  96   0  96  77   0   0   3   0  88   9  24  64   0   0   0   0  96   0   0
C |   3   0   0   0   1   0   0  81   0   0  25  24   7   0   0   0   0   0   0   0
G |  81   0  96   0  18  96  18   7  96   8   3  24  18  96   0  96  96   0  96  96
T |   3   0   0   0   0   0  78   5   0   0  59  24   7   0  96   0   0   0   0   0

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updated Fri Jan 9 10:35:13 2026