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structure nameISORETICULAR, INTERPENETRATING CO-CRYSTAL OF REPLICATION INITIATOR PROTEIN REPE54 AND SYMMETRICAL EXPANDED DUPLEX (31MER) CONTAINING THE COGNATE REPE54 SEQUENCE AND AN ADDITIONAL G-C RICH SEQUENCE WITH 2 STICKY BASE OVERHANGS AND 5' TERMINAL PHOSPHATES AND CROSSLINKED WITH EDC.
(Replication initiation protein)
referenceSlaughter et al.
sourceEscherichia coli K-12
experimentX-ray (resolution=3.24, R-factor=0.197)
structural superfamily"Winged helix" DNA-binding domain;
sequence familyInitiator Replication protein;
multimeric complexes2z9o_AB
reference complex8ac8_C
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureSAESKRRSYRPDRRR

Estimated binding specificities ?

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# IC=15.763 IC/col=0.751 n_of_columns=21 specificity:   
A |  64   0  24  10   0   0   0  83   0  96  24  24  24  24  10   0   0  24  91   5  91
C |  12  96  24  12   0   0   0   5  96   0  24  24  24  24   8  96  96  24   1  83   1
G |   8   0  24   8  96   0  96   4   0   0  24  24  24  24  66   0   0  24   0   4   4
T |  12   0  24  66   0  96   0   4   0   0  24  24  24  24  12   0   0  24   4   4   0
scan!

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updated Tue Dec 17 20:05:01 2024