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structure nameCRYSTAL STRUCTURE OF A TAILORED I-CREI HOMING ENDONUCLEASE PROTEIN (3115 VARIANT) IN COMPLEX WITH ITS TARGET DNA (HAEMOGLOBIN BETA SUBUNIT GENE) IN THE PRESENCE OF CALCIUM
(DNA ENDONUCLEASE I-CREI)
referenceMolina et al. 'Sci 8 10286 2018
sourceCHLAMYDOMONAS REINHARDTII
experimentX-ray (resolution=2.15, R-factor=0.180)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG endonuclease;
multimeric complexes6fb0_AB 6fb5_AB
redundant complexes6fb5_B
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureSIQKSKKTYSVNYKT

Estimated binding specificities ?

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# IC=6.708 IC/col=0.745 n_of_columns=9 specificity:   
A |  71   0  60  49   0  24   0   3  68
C |   3   0   3   5   0  24  96   3   3
G |  19  96   3  24  96  24   0   6  18
T |   3   0  30  18   0  24   0  84   7
scan!

Dendrogram of similar interfaces ?

matrix format
  
  SGIGQAKG----SC--KG----KGTA--YC--ST----VG--YA--KCTG   L                                      +6fb0_B    
                                                                                            +-1 
  SAIAQGKTNT--SCYAQA----QATCYARG--RG--SAVA------KGTC   L                                  +-2 +1t9i_B    
                                                                                          ! ! 
  STITQAKGRG--STNTQTST--ATTC--YT--SC----VCDTRGSTKGTG   L                              +---3 +3mxb_B    
                                                                                      !   ! 
  SGIGQAKGNC--SC--RGQG--DGTAYCAT--SC----VCKGRG----TT   L                        +----11   +6fb0_A    
                                                                                !     !  
  STITQANG----ST--RGRG--YCTCYCRG--------VCQT----KGTC   L                        !     !  +3mxb_R    
                                                                                !     +--8 
  SAYCLCITRGDCYA--QG------IG----RG--DT--IC--RG------   L                        !        +2fld_B    
                                                                                !  
  SGIGQAEC----STYARGKG--KGTA--RT--EC----VG------KTTG   L              +--------14             +2vbl_A    
                                                                      !         !           +-4 
  SAIAQAKG----ST--RG----QATAYCRT--ST----VA--IT--KTTT   L              !         !         +-5 +2vbo_A    
                                                                      !         !         ! ! 
  SAIAQA------SC--RGKG--QATAYC----ST----VA------KTTT   L              !         !  +------6 +2vbl_B    
                                                                      !         !  !      ! 
  STITQAKGRG--STNTQGST--ATTC--YT--SC----VCDTRGSTKGTG   L           +-15         +-12      +2xe0_B    
                                                                   !  !            !  
  STMG--------KGYCRT--RGVTSA----MC------STQTRGEC----   L           !  !            !     +5esp_A    
                                                                   !  !            +-----7 
  SA--RGRGNT--KTSTSTEC--QGTA----ST------AT--KG------   L           !  !                  +6bd0_A    
                                                              +---16  !  
  SAYCKTITRGDCYA----------IG----RG--DT--IC----IA----   L      !    !  +-------------------1m5x_B    
                                                              !    !  
  --MGTASG------RASTRGRGRG--------------DTRGRCEC----   L      !    !                  +6bce_A    
                                                              !    !          +------10  
  SGSTLGQG----KTYARGKGVT------HCHG--------QTRTDC----   L   +-17    !          !       +4yis_A    
                                                           !  !    +---------13  
  SCMCRGRG--------YTST--ECTT----RG------TTRG--------   L   !  !               !       +5thg_A    
                                                           !  !               +-------9 
  SC--YT------------RGKGTTTCIG--NA------AT--IC------   L -18  !                       +5t8d_A    
                                                           !  !  
  CA--QGVTRT--DCYA--RT----FA----RGNTDT--MCEA------KA   L   !  +------------------------5a77_B    
                                                           !  
  SAIAQAKGNT--SCYAQA----QATGYCRG--RG----VA--------TC   L   +------------------------1mow_A    
                                                         

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updated Fri Sep 13 21:38:32 2024