1fiu_B : 3D-footprint complex

structure name	TETRAMERIC RESTRICTION ENDONUCLEASE NGOMIV IN COMPLEX WITH CLEAVED DNA (TYPE II RESTRICTION ENZYME NGOMI)
reference	Sasnauskas et al. Nat.Struct.Biol. 7 792 2000
source	Neisseria gonorrhoeae
experiment	X-ray (resolution=1.60, R-factor=?)
structural superfamily	Restriction endonuclease-like;
sequence family	NgoMIV restriction enzyme;
multimeric complexes	1fiu_AB 1fiu_CD
redundant complexes	4abt_B
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 1fiu_B interface=34,36,63,191,192,193,194,227, mqplftqerrifhkklldgnilatnnrgvvsnaDgSntrsfniakgiadllhsetvserlpgQtsgnafeaicsefvqsafeklqhirpgdwnvkqvgsrnrleiaryqqyahltalakaaeenpelaaalgsdytitpdiivtrnliadaeinrneflvdeniatyaslragngnmpllhasisckwtiRSDRaqnarseglnlvrnrkgrlphivvvtaeptpsRissialgtgeidcvyhfalyeleqilqslnyedaldlfyimvngkrlkdisdlpldlav
interface signature	DSQRSDRR

Estimated binding specificities ?

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# IC=7.718 IC/col=0.965 n_of_columns=8

specificity:

A |   8   8   8   0   0   0   0   8
C |  64   8  64  96   0   0  96   0
G |  16  72   8   0  96  96   0  88
T |   8   8  16   0   0   0   0   0

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Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: NGOMIV
CCGGC	PubMed	1fiu_B(1.76e-06), 1fiu_AB(1.62e-06), 1fiu_CD(1.62e-06), ... (total=4)
G\|CCGGC	PubMed	1fiu_B(1.76e-04), 1fiu_AB(1.66e-04), 1fiu_CD(1.66e-04), ... (total=4)
GCCGGC	PubMed	1fiu_B(7.42e-09), 1fiu_AB(3.65e-09), 1fiu_CD(3.65e-09), ... (total=4)

Dendrogram of similar interfaces ?

matrix format

  
  DCSC------QC------------RGSGDCRG--RG   L              +------------------1fiu_B    
                                                 +------1 
  ----NGACKG------------SGRGSADCRG----         +-2      +------------------3dvo_D    
                                               ! ! 
  --------------SCNGNG--SGRG----RG----   L   +-3 +-------------------------3v1z_B    
                                             ! ! 
  ------------RG------------------VT--   L --4 +--------------------------3hxo_A    
                                             ! 
  --------------------RAKGRGECRG------   L   +---------------------------3bm3_A

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updated Tue Dec 19 05:37:44 2023