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structure nameSTRUCTURE OF CLOSTRIDIUM BUTYRICUM ARGONAUTE BOUND TO A GUIDE DNA (5' DEOXYCYTIDINE) AND A 19-MER TARGET DNA
(Clostridium butyricum Argonaute)
referenceVan der Oost et al. 'Nucleic 47 5809 2019
sourceClostridium butyricum
experimentX-ray (resolution=3.55, R-factor=0.250)
structural superfamilyRibonuclease H-like;
sequence familyPiwi domain;
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureNHQTRKTYYNERNK

Estimated binding specificities ?

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# IC=9.315 IC/col=0.621 n_of_columns=15 specificity:   
A |  96  24  24  96  11  24   9  24  14  24  96   9   4   0   0
C |   0  24  24   0  67  24   9  24  52  24   0  67  91   0  96
G |   0  24  24   0   9  24   9  24  14  24   0  11   0   0   0
T |   0  24  24   0   9  24  69  24  16  24   0   9   1  96   0
scan!

Dendrogram of similar interfaces ?

matrix format
  
  NA--HTQAYT--KANA------QARA----TGRA--KT----KTQTKTYTIC----NCYC------TG--LTYTYGHTNGEG--RANT--------------------KA------   L                      +--------6qzk_A    
                                                                                                                                   +------------1 
  ----------------LTPTYT----ITPT----LA------------------MT--RT--IT--------HCRG--NG--------SCDGVG--RGFGRG--RGFGAGFG----             !            +--------4n41_B    
                                                                                                                                   ! 
  --PC------WG--------------------KG----YAYARC------------------------SA--YA--------RC--DC--RA--KG--------SA--LAPA----   L   +-----5      +--------------6d92_F    
                                                                                                                             !     !    +-2 
  --------------------------------TT----------------------------------KCFC------------------------------LG--YCTC------   L   !     !  +-3 +--------------5ux0_A    
                                                                                                                             !     !  ! ! 
  --------------------------------------------------------------NT----------------------------------------------------     --6     +--4 +----------------5g5t_A    
                                                                                                                             !        ! 
  ----------------------------------------------------YA--TA--YA--RA------FAYTDTNT--------------------------FTRA------       !        +------------------6t5t_A    
                                                                                                                             ! 
  ----------------------------------------------------------------------------------------------------------------QGKG       +---------------------------2w36_A    
                                                                                                                           

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updated Fri Sep 13 23:16:47 2024