6uw0_B : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND FULLY ALTERED SPECIFICITY TARGETING CHOLERA TOXIN A SUBUNIT (I-ONUI-E-THERM-BCTXA)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.72, R-factor=0.228)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
reference complex	6uwj_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uw0_B interface=18,22,24,26,28,36,40,42,66,70,72,74,134,174,176,178,180,189,191,195,217,219,221,226,228,230,234, assinpwiltgfadaegSfglRiRkRnKssvgystElgfEiKlhnkdksileniqstwgvgvianSgsnAvRlRvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgegCfFvNlLksksklgvQvClvfSigqhirdknlmnslitylgcgYiLkKnkseFsWlEfcvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgr
interface signature	SRRRKEEKSARRWCFNLQCSYLKFWET

Estimated binding specificities ?

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# IC=22.849 IC/col=1.088 n_of_columns=21

specificity:

A |   0   0  11   0   0   0   0   0   0  62  24   0   0   0  86   0   0   0  81   4   7
C |   0   0   9  96  10   0   0   0  96   9  24   0  96   0  10  96   0   0   7   0   4
G |   0  96   9   0   0  96  96   0   0   9  24   0   0   0   0   0   0   0   4   0   4
T |  96   0  67   0  86   0   0  96   0  16  24  96   0  96   0   0  96  96   4  92  81

scan!

Dendrogram of similar interfaces ?

matrix format

  
  SA--RARGRGKT----EC--ECKG--STATRGRG--WGCAFT--NTLA------QACT----SA--YTLAKG----FTWCEA--TT   L                              +6uw0_B    
                                                                                                 +------------------------1 
  SGSTLGQG--KTYARGKGVT----HCHG--QTRTDC--SANA--KG--STTASARGQGRG----------YC--------RTEAVG   L   +-2                        +4yis_A    
                                                                                               ! ! 
  ------------------------------------------DT----------------------------NGKG----------   L --3 +-------------------------1r7m_B    
                                                                                               ! 
  ------------------------------------------------------------FC--AG--RC----------FA----   L   +---------------------------6dg0_A

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updated Fri Jan 9 10:34:21 2026