6uw0_B : 3D-footprint complex

structure name	ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE WITH IMPROVED THERMOSTABILITY AND FULLY ALTERED SPECIFICITY TARGETING CHOLERA TOXIN A SUBUNIT (I-ONUI-E-THERM-BCTXA)
reference	Stoddard et al. Structure 28 760 2020
source	SYNTHETIC CONSTRUCT
experiment	X-ray (resolution=2.72, R-factor=0.228)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 6uw0_B interface=18,22,24,26,28,36,40,42,66,70,72,74,134,174,176,178,180,189,191,195,217,219,221,226,228,230,234, assinpwiltgfadaegSfglRiRkRnKssvgystElgfEiKlhnkdksileniqstwgvgvianSgsnAvRlRvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehltiegikelvrikaklnWgltdelkkafpeiiskerslinknipnfkwlagftsgegCfFvNlLksksklgvQvClvfSigqhirdknlmnslitylgcgYiLkKnkseFsWlEfcvTkfsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgr
interface signature	SRRRKEEKSARRWCFNLQCSYLKFWET

Estimated binding specificities ?

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# IC=23.879 IC/col=1.085 n_of_columns=22

specificity:

A |  84  92   4  96  96   0   0  96   0  96  24  12   0  96   0   0  88   0  72   0  96  76
C |   4   0   4   0   0   0   0   0   0   0  24  12   0   0  96  96   0   0   8  96   0   8
G |   4   0   4   0   0  96   8   0  96   0  24   8  96   0   0   0   8  96   8   0   0   8
T |   4   4  84   0   0   0  88   0   0   0  24  64   0   0   0   0   0   0   8   0   0   4

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Dendrogram of similar interfaces ?

matrix format

  
  SA--RARGRGKT----EC--ECKG--STATRGRG--WGCAFT--NTLA------QACT----SA--YTLAKG----FTWCEA--TT   L                              +6uw0_B    
                                                                                                 +------------------------1 
  SGSTLGQG--KTYARGKGVT----HCHG--QTRTDC--SANA--KG--STTASARGQGRG----------YC--------RTEAVG   L   +-2                        +4yis_A    
                                                                                               ! ! 
  ------------------------------------------DT----------------------------NGKG----------   L --3 +-------------------------1r7m_B    
                                                                                               ! 
  ------------------------------------------------------------FC--AG--RC----------FA----   L   +---------------------------6dg0_A

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updated Sun Aug 3 13:33:45 2025