1fok_A : 3D-footprint complex

structure name	STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA (PROTEIN FOKI RESTRICTION ENDONUCLEASE)
reference	Schildkraut et al.
source	PLANOMICROBIUM OKEANOKOITES
experiment	X-ray (resolution=2.80, R-factor=0.214)
structural superfamily	Restriction endonuclease-like;"Winged helix" DNA-binding domain;
sequence family	Restriction endonuclease FokI, C termi; Restriction endonuclease FokI, recogni; Restriction endonuclease FokI, catalyt;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 1fok_A interface=9,10,76,92,100,101,102,214,215,217,219,222,225,226, kirtfgwvQNpgkfenlkrvvqvfdrnskvhnevknikiptlvkeskiqkelvaimnqhdliytykelvgtgtsiRseapcdaiiqatiadQgnkkgyiDNWssdgflrwahalgfieyinksdsfvitdvglaysksadgsaiekeilieaissyppairiltlledgqhltkfdlgknlgfsgesgftslpegilldtlanampkdkgeirNNwEgSsdKyaRMiggwldklglvkqgkkefiiptpdnkefishafkitgeglkvlrrakgsvpkrvywemlatnltdkeyvrtrralileilikagslkieqiqdnlkklgfdevietiendikglintgifieikgrfyqlkdhilqfvipnrgvtkqlvkseleekkselrhklkyvpheyielieiarnstqdrilemkvmeffmkvygyrgkhlggsrkpdgaiytvgspidygvivdtkaysggynlpigqademqryveenqtrnkhinpnewwkvypssvtefkflfvsghfkgnykaqltrlnhitncngavlsveelliggemikagtltleevrrkfnngeinf
interface signature	QNRQDNWNNESKRM

Estimated binding specificities ?

readout + contact

# IC=6.462 IC/col=0.923 n_of_columns=7

specificity:

A |  16   0  94   3   6   0   1
C |  20  92   1   2  89  95  14
G |  17   1   1   0   0   0  68
T |  43   3   0  91   1   1  13

scan!

Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: FOKI
GGATG	PubMed	1fok_A(7.08e-07)
CATCC	PubMed	1fok_A(7.08e-07)
CGATGC	PubMed	1fok_A(3.39e-03)
CATTC	PubMed	1fok_A(6.33e-03)
GGATGC	PubMed	1fok_A(6.29e-06)
CACCC	PubMed	1fok_A(5.92e-03)

Dendrogram of similar interfaces ?

matrix format

  
  QANARGQGDTNTWCNGNCECSAKGRGMG----------------------   L            +-------------------------1fok_A    
                                                                 +--4 
  ----------------------------------------YG--------             !  !                     +---3eyi_B    
                                                                 !  +---------------------2 
  --------------------------------------RCYG------KT             !                        +---2acj_B    
                                                               +-5 
  --------------------------------------NCITNARGRG--   L       ! !                            +6fqq_E    
                                                               ! !          +-----------------1 
  --------------------------------------NT--NAIARG--   L     +-6 +----------3                 +4cyc_B    
                                                             ! !            ! 
  ----------------------------RC--------IAQCNAYC----   L     ! !            +-----------------3rkq_B    
                                                           +-7 ! 
  --------------------------------------NCICNARGRG--   L   ! ! +-----------------------------4xrm_B    
                                                         --8 ! 
  ------------------------------------QTVAQTNA------   L   ! +-----------------------------8osb_E    
                                                           ! 
  ------------------------------TTKATTQTTGLTRT--KA--   L   +-----------------------------2p7c_B

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updated Mon Jun 29 14:12:32 2026