5e67_A : 3D-footprint complex

structure name	K103A/K262A DOUBLE MUTANT OF I-SMAMI (I-SmaMI LAGLIDADG meganuclease)
reference	Stoddard et al. J.Mol.Biol. 428 206 2016
source	Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) / Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)
experiment	X-ray (resolution=2.20, R-factor=0.183)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	4z1z_A 4z20_D 5e5o_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5e67_A interface=16,18,20,22,26,27,32,34,38,40,63,65,69,71,73,75,177,179,181,185,186,191,193,197,199,221,223,225,228,230,234, klnpwavvgfidaegSfMvRvRknsKYktgwLvVaifSvTvdkkdlflleslktffgglgsiKkSgnsTfSyRiEsseqltkiilpffdkyslitealgdyllfkkvlelmgtkehltqrglekivslkasinkglseelqaafpqcvptprpeinnknipdpfwlagfvsgdgsfKsIlKkseSIkvgfQsIlvfQiTqhardvklmeslisylgcgfiEkDsRgpWlYytvTnfsdiqgkiipffhqykiigsaygdyqdwckialimqnknhltpeglneiralkggmnkg
interface signature	SMRRKYLVSTKSTSREKIKSIQIQTEDRWYT

Estimated binding specificities ?

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# IC=21.843 IC/col=0.993 n_of_columns=22

specificity:

A |   5   5  96   0  96   0   0   0   0  74  24  24  24   5   0   0  96   0   0  96   7  96
C |  12   6   0  96   0  96  96   0   0  12  24  24  24   5   0   0   0   0   0   0  10   0
G |  69   5   0   0   0   0   0   0  96   5  24  24  24   5  96  96   0  96  96   0   5   0
T |  10  80   0   0   0   0   0  96   0   5  24  24  24  81   0   0   0   0   0   0  74   0

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Dendrogram of similar interfaces ?

matrix format

  
  SGMGRGRG--KTYALT--VT----TT----KT--SC----TA--RGEC----KGITKG--SGITQAICQATC--ECDTRG--WTYC--TC   L                               +5e67_A    
                                                                                                          +--------------------2 
  ST--SC--------HT--RT--TTMT----LT--NC----YA----YAWGSAFCRGRG------RGQGECSCYT------RTWCQTRTEC   L          !                    +7rcf_A    
                                                                                                   +------4 
  STITQA----ST--RG--RG--YCTCYC----RG------VCQT------STITQAKGRGSTNTQT--ATTC----YTSC--VCDTRGST   L   !      !      +--------------3mx9_A    
                                                                                                   !      +------3 
  CA--QGVTRTDCYA----RT----FG----RG--RG--DTMCEA----------------------------------------------   L --5             !              +5a78_B    
                                                                                                   !             +--------------1 
  STITQAKGRGSTNTQT--ST--ATTC----YT----SC--VCDTRGST------------------------------------------   L   !                            +3mxb_B    
                                                                                                   ! 
  --YTLC--------RGSGECRGVTTG--QG--VG------RTEARGSC------------------------------------------   L   +----------------------------5o6i_A

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updated Sat Jan 10 08:00:35 2026